Journal:
Article Title: p53-Dependent Elevation of p21 Waf1 Expression by UV Light Is Mediated through mRNA Stabilization and Involves a Vanadate-Sensitive Regulatory System
doi:
Figure Lengend Snippet: Effects of various inhibitory agents on p21 Waf1 expression in p53 −/− MEFs following mimosine treatment, UVC irradiation, or both a
Article Snippet: Fifty-microgram samples of total cell lysates were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes by using standard techniques. p21 Waf1 protein was detected with the ECL system (Amersham, Arlington Heights, Ill.) following incubation with a polyclonal rabbit anti-mouse p21 Waf1 antibody (PC55) from Calbiochem (Cambridge, Mass.).
Techniques: Expressing, Irradiation